ANTICONVULSANT STUDIES OF THE METHANOL EXTRACT AND FRACTIONS OF CASSIA SIAMEA LAM. (FABACEAE) IN MICE AND CHICKS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Cassia siamea is a shrub belonging to the Fabaceae family, native of Southeast Asia. The plant is commonly used in traditional medicine to treat hypertension, malaria and diabetes. Due to the easy cultivation of the plant, its widespread and also remarkable biological activities, Cassia siamea has become a worldwide medicine. The study was conducted to assess the anticonvulsant potential of the methanol extract of Cassia siamea (CSME) and its fractions (EAF, NBF and RAF) using Pentylenetetrazole (PTZ) induced seizures in mice and Maximal electroshock (MES) induced seizures in chicks. Acute toxicity was carried out on CSME and fractions. The possible mechanism(s) involved in anticonvulsant action were determined using Picrotoxin and naloxone. The preliminary phytochemical screening of the methanol extract revealed the presence of alkaloids, flavonoids, polyphenols, saponins, steroids, terpenoids and tannins. The LD50 of CSME, its EAF and NBF was found to be greater than 5000mg/kg intraperitoneally (i.p) in chicks and mice, suggesting a non toxic profile of the extractst, while the LD50 of the RAF (i.p) was found to be 1095mg/kg in mice and 3807 mg/kg in chicks implying the RAF is mildly toxic via the intraperitoneal route. The CSME and its EAF were found to have varied anticonvulsant activities; the EAF at 250mg/kg dose protected 40% of mice against hind limb tonic extension induced by maximal electroshock and in convulsed chicks a significant (P < 0.05) decrease in mean recovery time was noted. The ethyl acetate fraction at 250mg/kg and 500mg/kg doses produced a significant (P < 0.05) delay in mean onset of seizures and offered mice a 2/6 and 5/6 quantal protection against mortality, valproic acid the standard anticonvulsant drug used produced a 100% protection against seizures. The EAF did not protect mice against pirotoxin induced seizures indicating lack of activity on chloride ion channels of the GABAA receptor complex. Naloxone did not reverse the anticonvulsant activity of the EAF 2 against Pentylenetetrazole-induced seizures, suggesting lack ogf involvement of GABAABDZ receptors and opioid receptors in the anticonvulsant effect of EAF. The CSME and EAF at 100mg/kg dose significantly (P < 0.05) prolonged the total duration of Diazepam induced sleeping time in mice without affecting the mean onset of sleep, indicating sedative action of the extract. The results suggests the presence of bioactive component(s) that posess anticonvulsant and sedative activities. The data may provide pharmacological basis for the use of the plant alone or in combination with other plant(s) in the management of febrile convulsions and insomnia in West African countries including Nigeria.
Related Project Materials
CONSTRUCTION PROCEDURE IN CHEMISTRY OBJECTIVE TEST IN SENIOR SECONDARY SCHOOL
Abstract
This study is on construction procedure in chemistry objective test in secondary school. The total popula...
THE IMPACT OF EARLY CHILDHOOD EDUCATION ON ETHICAL DECISION-MAKING
ABSTRACT: Early childhood education (ECE) significantly impacts the development...
Rhetoric in African Fiction- a Postcolonial Analysis to Ngugi’s A Grain of Wheat, Petals of Blood, Devil on the Cross and Wizard of the Crow
Background to the Study
Rhetoric is the ancient art of argumentation and discourse from primordial Greece to the twentyfirst century. Rhe...
EARLY CHILDHOOD EDUCATION'S IMPACT ON COGNITIVE FLEXIBILITY
ABSTRACT: Early childhood education's impact on cognitive flexibility...
EFFECT OF HUMAN RELATIONS ON ORGANIZATIONAL PRODUCTIVITY
ABSTRACT
The study was designed to find out the “Effect of Human Relations on Organizational Productivity in N...
CAUSES AND EFFECTS OF TRUANCY ON PRIMARY SCHOOL PUPILS ACADEMIC PERFORMANCE IN MATHEMATICS
ABSTRACT
This project work is essentially concerned with the investigation of the study of truancy amon...
FACEBOOK AND POLITICAL MOBILIZATION ON OYO STATE: A CASE STUDY OF 2023 GENERAL ELECTIONS IN AKINLEYE LOCAL GOVERNMENT
Background of the study
According to Hot Mass Communication Topic (2023), newspapers, periodicals, radi...
THE INFLUENCE OF REWARD ADMINISTRATION ON TOTAL QUALITY MANAGEMENT IMPLEMENTATION: A STUDY OF CARITAS UNIVERSITY, AMORJI-NIKE ENUGU, ENUGU STATE, NIGERIA
ABSTRACT
Consequent upon titanic competition that has beclouded business environment of all sorts, organizations have employed myriad of...
AN ASSESSMENT OF INTERGOVERNMENTAL RELATIONS AND ITS IMPACT ON LOCAL GOVERNMENT ADMINISTRATION
EXCERPT FROM THE STUDY
Intergovernmental Relations is described as a network of institutions at the national, regional,...
PROBLEMS OF PROPERTY MANAGEMENT AND ITS SOLUTIONS IN NIGERIA
Abstract
Property management is a vital aspect of real estate profession. To a non-professional estate...
