EVALUATION OF BIOACTIVE METABOLITE CONTENTS, MOSQUITO LARVICIDAL AND ANTI-PLASMODIAL ACTIVITIES OF EXTRACTS OF THE SPIDER Neoscona adianta
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 5000
ABSTRACT
In spite of the extensive control efforts, over the years, mosquito still transmit serious human diseases, cause millions of deaths every year and the development of resistance to chemical insecticides resulting in rebounding vectorial capacity. This situation is aggravated with the re-emergence of drug resistance of mosquito borne diseases especially malaria. The current study was therefore designed to assess the mosquito larvicidal potency and antiplasmodial efficacy of the crude and fractions of the extract of the spider (Neoscona adianta). The zoochemical components of the crude and fractions of the spider extracts were assessed following standard procedures. The larvicidal potency of the crude and fractions of the spider was carried out according to World Health Organization (WHO) standard protocol with slight modifications. Graded concentrations (ranging from 0.2 to 2.0 mg/k) of the crude and fractions of the spider extracts were tested against 25 batches of healthy 4th instar larvae of Culex mosquito species, and larval mortality was recorded after 24 hours exposure period. Acute oral toxicity of the crude extract was carried out to establish the oral safe dose. The antiplasmodial activates of the crude extract and fractions were bio-assayed against established infection in chloroquine-sensitive Plasmodium berghei infected mice. The results indicated the presence of zoochemicals including flavonoid, tannins, saponnins, alkaloids steroids total phenol and terpeniods in the crude extract and fractions of the spider. The results of the larvicidal bio-assay revealed that both crude and fractions showed a dose and concentration-dependent larvicidal potency. Larvicidal activities was significantly higher (P<0.05) in the ethylacetate and n-henane fractions than in the crude methanol extract. Similarly, only the ethylacetate and n-hexane recorded 100% mortality for the highest concentration tested (2.0 mg/h). The best larvicidal activity was found in the nhexane fraction with an LC50 of 0.46mh/L, followed by ethylacetate with LC50 of 0.94mg/L. The results of the acute oral toxicity revealed that the spider extract is safe for oral administration with an LC50 greater than 5000mg/kg body weight. The crude and fractions of the spider extract showed a dose dependent antiplasmodial activities with peak activity recorded of the group of mice treated with 600mg/kg b.wt crude extract. The crude and fractions did not ameliorate fall in PCV but promoted body weight change and elongated the survival time. The results support the medicinal use of spider extracts in folkloric medicine and suggest that this spider contained bio-active compounds that could be developed as potent antimalarial drug as well as potent bio-pesticide agent against mosquito vector.
Related Project Materials
Spectrum Regulation Framework: A Case Study of NCC, Nigeria
ABSTRACT
Spectrum is a vital requisite for growth enhancement in this Digital Economy, and the efficient way of managing the use of the s...
An Evaluation of the Growth of Luxury Hotels in Abuja and Their Impact on the Tourism Sector
The expansion of luxury hotels in Abuja is a testament to the city's growing status as a key business and tourism hub in Nigeria. With its stat...
The impact of multilingual instruction on early childhood education language proficiency in Fagge Local Government Area, Kano State
Background of the Study
Multilingual instruction has emerged as a progressive educational practice, parti...
INEC AND CHALLENGES OF MONITORING POLITICAL PARTY CAMPAIGN FINANCING IN NIGERIA
Abstract
Democracy is a system in which the government is controlled by the people, and in which people are considered equals in the exer...
Public Perception of Excise Taxes on Imported Goods in Karu LGA
Background of the Study
Excise taxes on imported goods are commonly used by governments to generate rev...
An Appraisal of Geriatric Nurses’ Competency in Managing Polypharmacy in Kano State
Background of the Study
Polypharmacy, defined as the use of multiple medications by a...
Exploring the Use of Blockchain Technology for Certificate Verification in Secondary Schools in Gusau Local Government Area, Zamfara State
Background of the Study
Blockchain technology, known for its security, transparency, and decentralization, has gained wi...
Implementation of a Digital Twin System for University Infrastructure Management: A Case Study of Kano State University of Science and Technology, Wudil, Wudil LGA
Background of the Study
Managing university infrastructure efficiently is critical to ensuring a conducive learning enviro...
The impact of language contact on syntactic development among Yoruba and English bilinguals
Background of the study
Language contact occurs when speakers of different languages interact, often resulting in syntacti...
The effect of political violence on citizens' rights in Makurdi, Benue State.
Background of the Study:
Political violence, particularly during electoral periods, has been a persistent issue in Nigeria,...
