THE EFFECT OF METHANOL RHIZOME EXTRACT OF Nymphaea lotus LINN. (NYMPHAEACEAE) IN ANIMAL MODELS OF DIARRHOEA
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
Abstract
Nymphaea lotus is one of the foremost aquatic macrophytes that have been identified in Nigerian fresh water bodies. It finds applications in the management of circulatory system disorders, digestive system disorders, infectionsand inflammations. This study aims to evaluate the antidiarrhoeal activity of the methanol rhizome extract of N. lotus Linn plant in laboratory animals. Shade-dried rhizome of N. lotus was extracted with 80% methanol using Soxhlet apparatus. Preliminary phytochemical screening was carried out.This revealed the presence of alkaloids, anthraquinones, carbohydrates, cardiac glycosides, flavonoids, saponins, steroids, tannins and triterpenes. The oral median lethal dose (LD50) was determined andacute toxicity test of the extract gave LD50 value greater than 5,000mg/kg p.o. in mice. The antidiarrhoeal activityof the methanol extract was determined in mice. The extract was screened for activity against castor oil-induced diarrhoea and magnesium sulphate-induced diarrhoea as well as effect on gastric transit time in mice. For castor oil-induced diarrhoea, the extract at doses of 200, 400 and 800mg/kg produced statistical significant reduction in the frequency of diarrhoea (at p<0.001, p<0.001 and p<0.01 respectively). The extract at 800 mg/kg produced a significant delay in onset of diarrhoea (p<0.05) comparable to loperamide (3mg/kg). The frequency of magnesium sulphate-induced diarrhoeawas also significantly reduced in the groups treated with 200, 400 and 800mg/kg of the extract at p<0.001, p<0.001 and p<0.01 respectively. At doses of 200mg/kg (76.5%) and 400mg/kg (72.6%), the protection produced comparable to loperamide, 3mg/kg (70.6%).All treated groups produced statistically significant reduction in the transit of charcoal meal along the intestinal tract at p<0.001. The standard antidiarrhoeal drug, atropine (5mg/kg) produced greater antimotility effect (56.83%) vi compared to the extract. Effect of methanol rhizome extract of N. lotus on isolated rabbit jejunum and guinea pig ileum was determined. The methanol rhizome extract ofNymphaea lotus at low concentration (4×10-4 – 6.4×10-2mg/ml) had contractile effect on the tone of contraction of the rabbit jejunum while at higher concentrations (8×10-2 -512×10-2 mg/ml) produced significant reduction in the tone and rate of spontaneous contraction of rabbit jejunum. Interaction of the extract,at concentration that causes relaxation, with acetylcholine,attenuated the effect of the latter in a manner similar to atropine.The methanol rhizome extract of N. lotus at lower concentrations (4×10-4 to 2×10-2 mg/ml) has no effect on contraction of the guinea pig ileum while higher concentrations (4×10-2 - 512×10-2 mg/ml) produced significant relaxant activity on guinea pig ileum. Interaction of the extract with histamine attenuated the effect of the latter which may be as a result of physiologic antagonism.This study has shown that the methanol rhizome extract of N. lotus has antidiarrhoeal properties thus justifying its use by the local population for this purpose.
Related Project Materials
ASSESSMENT OF THE FACTORS RESPONSIBLE FOR CHILD ABUSE AND NEGLECT IN NIGERIA
ABSTRACT
The child abuse menace is as old as the birth of the first child, until very recently child maltreatment has ne...
INVESTIGATION OF OCCUPATIONAL SAFETY AND HEALTH(OSH) COMPLIANCE LEVEL IN ADDRESSING COVID 19 PANDEMIC IN THE INDUSTRIAL SECTOR
BACKGROUND OF THE STUDY
In December 2019, the global population will be awoken to an outbreak of a dise...
THE IMPACT OF ADVERTISING IN THE MARKETING OF HOTEL SERVICES IN ENUGU METROPOLIS (A CASE STUDY OF ZODIAC HOTEL LTD)
ABSTRACT
This research was carried to find out the “impact of advertising in marketing of hotel service in Enugu m...
THE IMPACT OF EFFECTIVE SOCIAL NETWORKING IN ACHIEVING ORGANIZATIONAL GOALS (A CASE STUDY OF GOURMET PIZZA COMPANY, ABUJA)
ABSTRACT
This study was carried out to examine the impact of effective social networking in achieving organizati...
ASSESSMENT OF GEOTHERMAL POTENTIAL IN PARTS OF BORNO BASIN NORTH-EAST NIGERIA USING AEROMAGNETIC AND AERORADIOMETRIC DATA
ABSTRACT
This research was aimed towards the assessment of geothermal potential part of Borno Basin north-east Nigeria using aeromagnetic...
THE CAUSES AND EFFECT OF INDISCIPLINE AMONG STUDENT IN PUBLIC SECONDARY SCHOOL: A CASE STUDY OF ESAN CENTRAL LOCAL GOVERNMENT AREA OF EDO STATE
ABSTRACT
The purpose of this study was to investigate the causes...
INFLUENNCE OF EDUCATIONAL BROADCASTING ON ACADEMIC PERFORMANCE SECONDARY SCHOOL STUDENTS
ABSTRACT
The study investigated the influence of educational broadcasting on academic performance of secondary schoo...
AN EXAMINATION ON A CRITIQUE OF DAVID HUME’S EMPIRICISM
BACKGROUND OF THE STUDY
The pursuit of knowledge that is unchangeable and unquestionable has been ongoi...
STAKEHOLDERS’ PERCEPTIONS ON PRINCIPALS’ LEADERSHIP STYLE PRACTICES IN SECONDARY SCHOOLS IN NORTH CENTRAL GEOGRAPHICAL ZONE, NIGERIA
ABSTRACT
This study investigated Stakeholders’ Perception onPrincipals’ Leadership StylePractices in Secondary Schools in the...
EFFECTS OF FUNDING, STAFF TRAINING, PRESERVATION AND BIBLIOGRAPHIC CONTROL ON NEWSPAPER UTILISATION IN UNIVERSITY LIBRARIES IN NIGERIA
ABSTRACT
Newspapers are sources of information on topical and current issues, stocked by libraries for use but due to their nature, they...
