STUDIES ON IN-VIVO ANTIOXIDANT AND HEPATOPROTECTIVE PROPERTIES OF CITRUS SINENSIS (L.) OSBECK PEEL EXTRACT IN WISTAR RATS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Citrus sinensis is the commonest, most widely cultivated and consumed citrus fruit. Large amounts of peel wastes are formed when citrus is consumed or processed; and suitable methods have to be adopted to utilize these waste materials for conversion into value-added products. Flavonoids, the major component of citrus peel have been reported to exert antioxidant and hepatoproective activity. This study was designed to investigate the in-vivo antioxidant and hepatoprotective properties of the methanol extract of citrus fruit peel in Wistar rats. Qualitative and quantitative phytochemical screening was carried out on extracts of three different citrus species fruit peels for comparison and selection, after which C. sinensis fruit peel extract was selected for further studies because it had the highest flavonoid content. Oxidative stress and liver damage was induced in rats using 2 ml/kg subcutaneous injection of CCl4 every 72 h for 10 days. Biochemical parameters were assessed as indices of oxidative stress and liver damage. Acute toxicity studies suggests that the LD50 (oral route) is above 5,000 mg/kg. The extract showed significant protection (at P<0.001) against the depletion of liver tissue catalase levels observed in the CCl4 treated group at 500 mg/kg (89% protection) comparable to that of the standard drug sylimarin (84% protection). The extract reduced liver enzymes significantly (at P<0.001 for alanine aminotransferase and aspartate aminotransferase; and P<0.05 for alkaline phosphatase as well as P<0.05 for bilirubin) similar to sylimarin. Furthermore, the extract also had similar protective effect to sylimarin on oxidative stress induced ischemia in kidney tissues. Urea levels were significantly (P<0.05) reduced from 6.86±0.80 mmol/L observed in the CCl4 treated group to 3.72±0.58 mmol/L by the 500 mg/kg extract. Preliminary phytochemical screening of methanol extract of C. sinensis fruit peels revealed the presence of flavonoids, phenols, tannins, saponins, steroids, carbohydrates and cardiac glycosides; with total flavonoids and phenol content of 17.3 mg QE/g and 13.6 mg GAE/g respectively. The pathological evaluations of liver sections of rats treated with C. sinensis peel extract showed mild distortion revealing less damage comparable to the control and sylimarin treated groups. Thus, it can be concluded that the methanol extract of C. sinensis peel is relatively safe orally in rats and possess antioxidant activity that may be effective in the management of liver damage and possibly other oxidative stress induced diseases. Thus, supporting the development of the biologically active compounds from C. sinensis as antioxidants for the management of hepatic disorders.
Related Project Materials
FINANCIAL MANAGEMENT STRATEGIES IN THE HEALTHCARE INDUSTRY
Abstract: FINANCIAL MANAGEMENT STRATEGIES IN THE HEALTHCARE INDUSTRY
This research explores financial management strategies in the health...
STUDY ON THE EFFICACY AND SAFETY OF ARTEMETHER/LUMEFANTRINE IN THE TREATMENT OF UNCOMPLICATED PLASMODIUM FALCIPARUM MALARIA AMONGST CHILDREN IN NIGERIA
ABSTRACT
Globally, malaria is a major health concern because it causes high morbidity and mortality particularly in children under 5 year...
AN ASSESSMENT OF UNIVERSITY MONITORING AND ITS IMPACT ON STUDENTS QUALITY OF EDUCATION (CASE STUDY OF NUC, ABUJA)
Background Of The Study
The importance of quality education at academic institutions cannot be overstat...
ASSESSING THE ROLE OF EARLY CHILDHOOD EDUCATION IN BUILDING COMMUNITY RESILIENCE
ABSTRACT: Assessing the role of early childhood education in building comm...
ASSESSMENT OF THE INTEGRATION OF SUSTAINABILITY EDUCATION INTO EARLY CHILDHOOD EDUCATION (ECE) PROGRAMS IN NIGERIA AND ITS IMPACT ON CHILDREN'S ENVIRONMENTAL AWARENESS, ATTITUDES, AND BEHAVIORS.
Abstract: This study explores the integration of sustainability education into early childhood education (EC...
EFFECTS OF FINANCIAL DEEPENING ON ECONOMIC GROWTH: EVIDENCE FROM NIGERIA
ABSTRACT
The design of proposed Modern Bus Terminal at Obalende employs the use of thin-shell concrete structures in order to provide max...
FRAMEWORK FOR THE PROCUREMENT OF FACILITIES MANAGEMENT SERVICES OF PUBLIC BUILDINGS IN FCT-ABUJA
ABSTRACT
Deciding what to provide in-house and what to outsource is not always easy, because of the pros and cons of each approach. This...
SME FINANCING AND ECONOMIC GROWTH IN NIGERIA
Abstract
This research work examined the effect of SME financing and economic growth in Nigeria. The research was d...
THE SOCIO-ECONOMIC CORRELATION OF FEMALE CRIMINALITY IN NIGERIA [A CASE STUDY OF KADUNA PRISON]
ABSTRACT
The general objective of the study was to describe the extent and type of female involvement in crime and analy...
THE EFFECTIVENESS OF APPRENTICESHIP TAX INCENTIVES IN VOCATIONAL SECTORS
ABSTRACT: This study investigates the effectiveness of apprenticeship tax incentives in vocational sectors, emphasizing their role in encourag...
