PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES ON THE STEM-BARK OF COMMIPHORA MOLLIS (Oliv. ) Engl. (BURSERACAEA)
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Commiphora mollis is traditionally used in the treatment of fever (malaria and typhoid) wound healing, cancer, ulcer and rheumatic condition. The plant grows in Nigeria and across Africa. Phytochemical analysis of methanol extract of the stem-bark of Commiphora mollis showed the presence of flavonoids, saponins, tannins, terpenoids and alkaloids. Extensive Phytochemical studies of ethylacetate water soluble fraction of methanol extract resulted in isolation (55 fractions, 100ml each were collected and pooled together based on similarities in their TLC profile to gave 8 major fractions and repeated gel filtration chromatography of Fraction 5 on Sephadex LH-20 packed column then preparative gave 7.5 mg of compound X1) and characterization using 1HNMR (9 proton signal 5 aromatic proton δ 7.0- 5.94 ppm, 2 oxymithine protons at 4.8 and 4.2 ppm and 2 methylene protons at 2.8 and 2.75 ppm), 13CNMR (15 carbon signals 12 in aromatic region, 2 oxymethine carbon and 1 methylene carbon.), DEPT (7 methine 1 methylene 1 quatenery ), 1H 1H COSY ( correlation between protons at 7.0, H2’ // 6.83, H6’ 7.0, H2’ // 4.8, H2 6.83, H6’ // 4.8, H2 4.8, H2 // 4.2, H3 4.8, H2 // 2.8, H4 4.2 , H3 // 2.8, H4 ), HSQC (correlation between proton and carbon at 7.0, H2’ // 114, C2’ 6.83, H6’ // 118.10, C6’ 6.8, H5’ // 114.60, C5’ 5.8, H6 // 94, C6 4.82, H2 // 78.48, C2 4.21, H3 // 66.10, C3 2.8, H4 and 2.7, H4 // 27.84, C4 ), HMBC and NOESY. The antimicrobial studies of the crude methanol extract, Chloroform and ethylacetate fractions were carried out using disc diffusion and broth agar dilution methods on clinical isolates of Corynebacterium ulcerans, Salmonella typhi, Proteus mirabilis, Bacillus sabtilis, Staphylococcus aureus, Streptococcus pyogens, Escherichia coli, Candida brusei, Shigella dysenteriae and Candida albicans. The crude methanol extract, chloroform and ethylacetate fractions showed strong inhibitory activity against all tested microoganisms with exception of Corynebacterium ulcerans, Salmonella typhi and Proteus mirabilis. Crude methanol extract was found to have MIC at 10 mg/ml for all organisms and vi variable value of MBC/MFC and that of chloroform and ethylacetate fractions have variable value for both MIC and MBC. The stem-bark of Commiphora mollis was rich in bioactive phytochemicals which have antimicrobial activity and could serve as a potential source of compounds effective against disease causing micro- organisms.
Related Project Materials
IMPACT OF NON-OIL EXPORT ON NIGERIAN ECONOMY (1986-2010)
ABSTRACT
The study investigated the impact of non-oil exports on Nigerian economy during the period of 1986-2010. This study was carried...
PROBLEMS OF FEMALE SECRETARIES IN BUSINESS ORGANIZATIONS (A Case Of NEPA)
ABSTRACT
This research work is aimed at defining the PROBLEMS OF FEMALE SECRETARIES IN BUSINESS ORGANIZATIONS A CASE OF...
DETERMINING THE SERUM PROTEIN LEVELS AND CIC LEVEL IN CEREBRAL MALARIA PATIENTS
OBJECTIVES OF THIS STUDY
This study is aimed at:
- Determining the serum protein levels and CIC level i...
Read more
THE RELEVANCE OF SALES PROMOTION TO BUSINESS ORGANIZATIONS
ABSTRACT
The study examined the relevance of sales promotion to business organizations using Sino Food Nigeria Ltd...
Read morePHYTOCHEMICAL AND ANTI-INFLAMMATORY STUDIES ON THE HEXANE EXTRACT OF THE STEM BARK OF STEGANOTAENIA ARALIACEAHOSCHTS (APIACEAE)
ABSTRACT
Inflammation has been implicated in virtually all human and animal diseases. It has become the focus of global scientific resear...
Read moreEFFECT OF INFORMATION COMMUNICATION TECHNOLOGY ON EARLY CHILDHOOD EDUCATION DELIVERY
ABSTRACT
The study attempted to investigate the impact of information communication technology (ICT) on educational deve...
Read moreTHE EFFECT OF MONETARY POLICIES OF THE CENTRAL BANK OF NIGERIA (CBN) ON DEPOSIT MONEY BANK
ABSTRACT
The role of Central Bank of Nigeria as the apex regulatory authority in the financial system...
Read moreLECTURERS’ AWARENESS, READINESS, SELF-EFFICACY AND INTENTION TO ADOPT MICRO-BLOGS FOR TEACHING AMONG UNIVERSITIES IN KADUNA STATE, NIGERIA
ABSTRACT
This study was designed to investigate lecturer‟s awareness, readiness and level of self-efficacy as determinant of micro-blogs...
Read moreimpact of intergrated marketing communication(inc)
Abstract
This study was informed by the necessity of research in the impact of integrated marketing communication on brand building. A st...
Read moreAN ASSESSMENT OF GOVERNMENT POLICIES AND PROGRAMMES IMPLEMENTATION STRATEGY
ABSTRACT
This study focused on the assessment of government policies and programme implementation strategies and the ext...
Read more
