EFFECTS OF DETOXIFICATION ON SOME PHARMACOLOGICAL PROPERTIES OF JATROPHA CURCAS LINN. (EUPHORBIACEAE) SEED OIL IN LABORATORY ANIMALS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
Abstract
Jatropha curcas seed oil is a known energy source in biodiesel which but for its toxicity, also has potential of being used as food and in the pharmaceutical / cosmetic industries. This study was designed to detoxify solvent and mechanically extracted J. curcas oils and to ascertain the effects of detoxification on acute and sub-acute toxicities as well as on analgesic and laxative activities in laboratory animals. Following determination of the physicochemical properties of the extracted oils, detoxification of the oils was attempted by both the usual synthetic method of orthophosphoric acid degumming, sodium hydroxide deacidification, tonsil decolourisation and vacuum-heat deodorization; and by the use of natural materials in place of the synthetic agents such as distilled water for orthophosphoric acid, plantain peel ash extract for sodium hydroxide and bentonite for tonsil. Phytoconstituents, elemental and phorbol esters compositions of the oils were also determined. Acute toxicity studies for LD50 determination and sub-acute (14 days) studies for toxicity symptoms of the mechanically extracted oil in male rats were carried out. Weight changes, mortality, haematological liver / kidney assessment as well as histopathological analyses of liver, kidney and intestines were checked. Wherever appropriate olive oil was used as vehicular control. Statistical analyses were by ANOVA with appropriate post hoc tests. The extraction yields of the oils were 49.1% (petroleum ether solvent) and 14.9% (hand operated hydraulic screw press). The physicochemical properties of the extracted oils were similar. Alkaloids were the only phytoconstituent present in both the undetoxified and detoxified oils. Detoxification resulted in oil losses upward of 60%, but with reasonable amounts of formed soaps (fatty-acid precipitate). Elemental analyses showed higher toxic elements (lead, cadmium and copper) and nutritional elements (iron, calcium and zinc) in the undetoxified oils compared to detoxified. The level of phorbol ester rich fraction of solvent extracted oil detoxified with synthetic materials was significantly (p0.05) for mechanically extracted oils detoxified either with synthetic or more natural materials. The oral LD50 of the detoxified oils of both extraction processes in both 3-day old cockerels and adult male mice were above 5,000 mg/kg. Sub-acute oral treatment with 125, 250 and 500 mg/kg of mechanically extracted oil resulted in dose-dependent toxic symptoms of ruffled fur, hyperpnoea, restlessness and decreased mobility mostly of the vii undetoxified (moderate to severe) as opposed to the detoxified oil (mostly mild). The rats that received the undetoxified oil suffered weight loss and dose-dependent mortality, but there was neither weight loss nor mortality in rats treated with the detoxified oil. Administration of the undetoxified oil caused significant (p0.05) for any of the oils. Histopathological examinations of the liver, kidney and intestines indicated a dosedependent moderate to severe necrosis and degeneration of tissues from the undetoxified oil, while detoxified oil produced mild degeneration of tissues. But for the high oil losses in the process of detoxification, the detoxified oil, based on the various indices studied, ameliorated the toxic effects of J. curcas oil and revealed its potential for use medicinally.
Related Project Materials
DESIGN OF A COMPUTER ASSISTED MULTIPLE CHOICE EXAMINATION SYSTEM (A CASE STUDY OF COMPUTER SCIENCE DEPARTMENT FEDPONEK OWERRI)
ABSTRACT
With the thoughtful application of emerging technologies and in a bid to improve on the effort tow...
INDECENT DRESSING AMONG UNDERGRADUATES: A CASE STUDY OF THE COLLEGE OF EDUCATION, EKIADOLOR, EDO STATE
ABSTRACT
Indecent dressing like other social vices has eaten deep into the lives of the youths, especia...
INVESTIGATION ON THE RISK ATTACHED TO USING E-NAIRA IN NIGERIA
Background of the study
Connection to the financial system is very important to different economies in...
STRATEGIES TO IMPROVE DELTA STATE TECHNICAL COLLEGES STUDENTS SKILLS AND ACADEMIC PERFORMANCE IN METALWORK TECHNOLOGY
ABSTRACT
This study was conducted to identify the strategies to improve Delta State Technical colleges students skills and academic perfo...
RECYCLING AND REUSE: ALTERNATIVES TO WASTE MANAGEMENT IN NIGERIA
Abstract
Waste is a global environmental issue that is becoming most noted in developing countries. Pu...
THE EFFECT OF COST ACCOUNTING ON INVENTORY VALUATION AND CONTROL IN REAL ESTATE
Abstract: THE EFFECT OF COST ACCOUNTING ON INVENTORY VALUATION AND CONTROL IN REAL ESTATE
This study examines the effect of cost accounti...
STRATEGIES FOR PROMOTING CAREER READINESS AMONG VOCATIONAL STUDENTS
ABSTRACT: This research examines strategies for promoting career readiness among vocational...
THE IMPACT OF INFORMATION AND COMPUTER TECHNOLOGY ICT ON THE MARKETING OF AUTOMOBILE PRODUCTS IN NIGERIA
BACKGROUND OF THE STUDY
As to the review of information technology, it combines the collection, gatheri...
ASSESSING THE BENEFITS OF EARLY CHILDHOOD EDUCATION IN DISASTER PREPAREDNESS
ABSTRACT: This study Assessed the Benefits of Early Childhood Education in Disaster Preparedness. The object...
ASSESSMENT OF PRIMARY EDUCATION STANDARD AND THE EDUCATIONAL GROWTH IN NASARAWA STATE
Abstract
The study examines primary education standard and the educational growth in Nasarawa state. The specific object...
