PHYTOCHEMICAL AND SOME PHARMACOLOGICAL STUDIES OF THE LEAF, STEM BARK AND ROOT BARK EXTRACTS OF STACHYTARPHETA ANGUSTIFOLIA (MILL) VAHL. (VERBENACEAE
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
The extracts of the leaf, stem bark and root bark of Stachytarpheta angustifolia (Mill) Vahl. (Verbenaceae), were subjected to preliminary phytochemical screening and the result revealed the presence of carbohydrates, steroids, cardiac glycosides, terpenoids, flavonoids, tannins and Saponins. Silica gel column chromatography of n- butanol soluble fractions of the leaf, stem bark and root bark extract followed by gel filtration using sephadex LH20 and preparative thin layer chromatography resulted in the isolation of two iridoid glycosides 1-[3,4-dihydroxy-5-(3-hydroxy-cycloprop-1-enyloxy)-6-hydroxymethyltetrahydro- pyran- 2-yloxy]- 7-methyl-1, 4a, 5, 6, 7, 7a hexahydrocyclopenta [c] pyran -4- carboxylic methyl ester and 4a, 7 – dihydroxy – 7 – methyl – 1 – (3, 4, 5 – trihydroxy – 6– hydro methyl – tetrahydropyran – 2 – yloxy) – 1 -, 4a, 5, 6, 7, 7a, hexahydro – cyclopentano [C] pyran – 4 – carboxylic acid methyl ester from the leaf and root bark respectively, while -β – (3‟, 4‟ – dihydroxy phenyl) – ethyl – O - - L – rhamnopyranosyl (1 3) – β – D – (4 – O - Caffeoyl) – glucopyranoside was isolated from the stem bark. The structures of these compounds were elucidated using chemical tests, spectroscopic techniques (FTIR, ID and 2D NMR) and by comparison with reference data. The ethanol leaf extract and its n-butanol and residual aqueous fractions exhibited concentration dependent contraction of the rabbit jejunum which was blocked by atropine, suggesting that the observed pharmacological effect were mediated through muscarinic receptors. In contrast, chloroform and Ethylacetate fraction of the ethanolic root bark extract exhibit concentration dependent relaxation of the rabbit jejunum. The ethanol leaf extract was screened for antimicrobial activity using disc diffusion and agar well dilution methods. The organisms tested were clinical isolates of S. aureus, S. pyogenes, E. coli and S. typhi. The ethanol extract inhibited the growth of all the microbes. The zone of inhibition ranging from 10-30mm). Conclusively, the use of S. angustifolia as a remedy for venerable infection by the folkloric healers has scientific basis .
Related Project Materials
THE EFFECTS OF EARLY CHILDHOOD EDUCATION ON CULTURAL SENSITIVITY IN DIVERSE COMMUNITIES
ABSTRACT: The effects of early childhood education on cultural sensi...
PERCEPTION OF INTERNET USERS ON DATA DRIVEN ADVERTISING AMONGST UNIVERSITY STUDENTS
ABSTRACT
As a result of new and improved technologies and techniques, advertising has witnessed...
iSight: An Object Recognition Application for Visually Impaired Individuals
ABSTRACT
Assistive technologies to aid the visually impaired have evolved over time from screenreading software, magnification programs a...
COMPARATIVE EFFECT OF CO-OPERATIVE LEARNING STRATEGY AND INQUIRY BASED METHOD
Abstract
Science may be viewed as a way of questioning or verifying the truthfulness of knowled...
IMPACT OF TRANSPORTATION ON ECONOMIC GROWTH: AN ASSESSMENT OF ROAD AND RAIL TRANSPORT SYSTEMS IN LAGOS STATE
ABSTRACT
The study looked at impact of transportation...
AN INVESTIGATION OF THE PHYSIOCHEMICAL PROPERTIES IN SACHET WATER IN ABA
BACKGROUND OF STUDY
Water is a liquid that has no taste, no smell, no color, and is completely transparent. It is...
MARITAL RIGHTS AND OBLIGATIONS IN ISLAMIC LAW: A CRITICAL ANALYSIS OF ITS PRACTICE IN NORTHERN NIGERIA
ABSTRACT
Marriage under Islamic law, as an institute is generally the cradle of any legitimate family and society. Hence the success of m...
THE PROBLEM OF TEACHING AND LEARNING OF HOME ECONOMICS IN TERTIARY INSTITUTION
ABSTRACT
The main focus of this study is to examine the problem of effective teaching and learn...
FACTORS THAT INFLUENCE THE UTILIZATION OF MATERNAL HEALTH CARE CENTERS, A CASE STUDY OF JABI VILLAGE FCT ABUJA
Background Of The Study
In the child of bringing new life into the world, childbearing is one of the pe...
A HISTORICAL ANALYSIS OF NATIONAL ECONOMIC EMPOWERMENT AND DEVELOPMENT STRATEGY (NEEDS) AND HER CONTRIBUTION TO POVERTY ALLEVIATION IN NIGERIA
INTRODUCTION
National Economic Empowerment Development strategy (NEEDS) is described as a Nigeria’s plan for prosp...
