ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM MASTITIC COWS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
This study investigated the antimicrobial susceptibility and molecular characterization of Staphylococcus aureus in mastitic cows in southern part of Kano State of Nigeria. A total of two hundred and thirty four (234) milk and swab samples were collected from six (6) local government areas of Kano State in northern part of Nigeria. Staphylococcus aureus was isolated from samples and identified using standard microbiological procedures. The antimicrobial susceptibility of the isolates was determined using Kirby- Bauer disc diffusion technique as described by the Clinical and Laboratory standards institute (CLSI). Resistant isolates were tested for Beta-lactamase production. A total of 200 (85.5%) have staphylococcal isolates, of which 141 (70.5%) were Staphylococcus aureus (coagulase positive staphylococci, CPS), 59 (29.5%) were coagulase negative staphylococci (CoNS) and 6 (2.9%) were non- Staphylococcal (rods) isolates. The antibiotic susceptibilities of the Staphylococcus aureus isolates were in the following order: ofloxacin (99.3%) > ciprofloxacin (98.6%) > gentamicin (91.5%) > cephalexine (56%) > sulphamethoxazole/trimethoprim (46.1%) > tetracycline (43.3%) > cefuroxime (33.3%) > cefoxitin (2.1%) > amoxicillin (1.4%) > ampicillin (1.4%). Resistance to three or more antimicrobials was presented in 98.6% of the Staphylococcus aureus isolates. Of the 141 Staphylococcus aureus isolates tested for β-lactamase production, 31 (22.5%) were β lactamase producers. However, of the 31 β-lactamase producers, only 13 (41.9%) were multidrug resistant to cefoxitin, Ampicillin and cephalexine. PCR assay was used to detect mecA and blaZ genes in multi-drug resistant Staphylococcus aureus isolates that were β-lactamase producers. The MDRStaphylococcus aureus was also tested by latex agglutination for presence of PBP2a. PCR results indicated none of the isolates showed any amplification to both mecA gene and blaZ gene. All the Beta-lactamase producing MDR- Staphylococcus aureus isolates tested showed negative reaction to latex agglutination test which was also an indication of absence of Pinicillin binding protein (PBP2a). In conclusion, high isolation of Staphylococcus aureus (70.5%) causing bovine mastitis was observed in this study in which both mecA and blaZ genes were absent
Related Project Materials
AN ASSESSMENT OF THE PRINT MEDIA IN THE CAMPAIGN AGAINST DRUG ABUSE IN NIGERIA
BACKGROUND OF THE STUDY
Drug abuse is a very sensitive and vital issue on our social, education, economic and moral life...
ASSESSMENT OF KNOWLEDGE AND ATTITUDE OF TEACHERS TOWARDS SAFETY AND SECURITY OF SECONDARY SCHOOL STUDENTS IN NIGERIA
ABSTRACT
The purpose of this study was to assess the knowledge and attitude of teachers towards safety and security of secondary school s...
ASSESSING THE IMPORTANCE AND EFFECT OF FIRST AID IN ROAD TRAFFIC ACCIDENT A CASE STUDY OF ACCIDENT AND EMERGENCY UNIT FMC ABEOKUTA OGUN STATE
ABSTRACT
This study assessed the importance and effect of first aid in road traffic accident. It sought to determine the...
IMPACT OF COMPUTER STUDY ON BUSINESS EDUCATION
Background of the study
In 1949 the government of Nigeria appointed F.J. Harlow, principal of Chelsea Polytechnic (Londo...
AN ASSESSMENT OF THE CAUSES OF ROAD ACCIDENT
EXCERPT FROM THE STUDY
In the light of the analysis carried out, the following conclusions were drawn.
- Mechanical...
Read more
DESIGN AND IMPLEMENTATION OF AN ON-LINE VOTING SYSTEM (A CASE STUDY OF ENSIEC ENUGU)
ABSTRACT
Voting is a very important process in the Nigerian political system. It is one of the means and most effe...
Read moreEFFECT OF COMPUTER ASSISTED INSTRUCTION (CAI) ON STUDENTS’ ACADEMIC ACHIEVEMENT IN GOVERNMENT
Background to the Study
The advancement in science and technology and their application in education ha...
Read moreAE-FUNAI UNDERGRADUATES STUDENT AWARENESS AND PERCEPTION OF POLICE BRUTALITY IN NIGERIA
BACKGROUND OF THE STUDY
The police are state personnel who are in charge of 'law enforcement and or...
Read moreSOCIAL MEDIA ADVERTISING AND ITS INFLUENCE ON AUDIENCE PREFERENCE OF SIMILAR PRODUCT
ABSTRACT
This study was carried out to examine social media advertising and its influence on audience preference of simi...
Read moreJOURNALISTS' PERCEPTION OF THE CREDIBILITY OF CITIZEN JOURNALISM ON SOCIAL MEDIA PLATFORMS
ABSTRACT
This study focused on journalists' perception of the credibility of citizen journalism on social media plat...
Read more
