ENUMERATION AND IDENTIFICATION OF BACTERIA ON USED HANDKERCHIEFS IN MALES
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ABSTRACT
This study was carried out on the enumeration and identification of bacteria on used handkerchiefs in males. The study was carried out in Makurdi town. Study was conducted on four samples used male handkerchiefs. (Sample A, Sample B, Sample C, Sample D Handkerchiefs). Each sample was collected in isolation in sterile polythene bags to avoid cross contamination. The identification of bacteria was based on their morphological and biochemical characteristics using procedure described by Cheesbrough, 2000. Microorganisms were gram stained, Air dried and viewed under microscope under x10 objectives. Growth on the sabouraud dextrose agar plates were observed for colour, pigment production, size of growth opaquecity. Data was analyzed using the descriptive statistic SPSS (version 20). The sample in this study were contaminated with the following contaminant for bacteria they were all colonized by one type of bacteria, Bacillus substilis. Fungal includes Candida albican, Candida tropicalis, Penicillium spp, Rhodotorula rubra, Trichophyton rubrum. This study has shown that used male handkerchiefs are colonized with one type of bacterial, Bacillus substilis. From this study, it is concluded that Sample B and Sample C have the highest percentage in fungal and all the handkerchiefs have the same percentages in bacteria isolates.
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